Restriction enzymes
Enzyme Finder, New England Biolabs http://www.neb.com/nebecomm/EnzymeFinder.asp
Harvard map translate http://pga.mgh.harvard.edu/web_apps/web_map/start
NEBcutter http://tools.neb.com/NEBcutter2/index.php
REBASE http://rebase.neb.com/rebase/
REBASE http://rebase.neb.com/rebase/rebase.html
Restriction enzymes http://www.mekentosj.com/enzymex/
Restriction Mapper http://www.restrictionmapper.org/
The Primer Generator http://www.med.jhu.edu/medcenter/primer/primer.cgi
WatCut http://watcut.uwaterloo.ca/watcut/watcut/template.php?act=set_preferences
WatCut - Restriction Analysis http://watcut.uwaterloo.ca/watcut/watcut/template.php
Webcutter   http://www.firstmarket.com/cutter/cut2.html
Webcutter 2.0 http://rna.lundberg.gu.se/cutter2/